Bacterial Artificial Chromosomes: Volume 1: Library Construction, Physical Mapping, and Sequencing Tapa dura - 2004
de Shaying Zhao (Editor); Marvin Stodolsky (Editor)
Primera línea
DNA cloning, especially large DNA cloning, is the first step in contemporary complex genome analysis.
Descripción de contraportada
The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1: Library Construction, Physical Mapping, and Sequencing presents readily reproducible techniques for library construction, physical mapping, and sequencing. The laboratory protocols follow the successful Methods in Molecular Biology(TM) series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms.
Comprehensive and cutting-edge, the two volumes of Bacterial Artificial Chromosomes provide a superlative collection of highly productive protocols that will prove useful to many bioscientists, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of specific genomes.
Detalles
- Título Bacterial Artificial Chromosomes: Volume 1: Library Construction, Physical Mapping, and Sequencing
- Autor Shaying Zhao (Editor); Marvin Stodolsky (Editor)
- Encuadernación Tapa dura
- Edición 1st
- Páginas 360
- Volúmenes 1
- Idioma ENG
- Editorial Humana, Totowa, NJ
- Fecha de publicación 2004-03-04
- Ilustrado Sí
- ISBN 9780896039889 / 0896039889
- Peso 1.67 libras (0.76 kg)
- Dimensiones 9.28 x 6.12 x 1.16 pulgadas (23.57 x 15.54 x 2.95 cm)
- Library of Congress subjects Chromosome Mapping, Cloning, Molecular
- Número de catálogo de la Librería del Congreso de EEUU 2003023009
- Dewey Decimal Code 660.65
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