G Protein Signaling: Methods and Protocols Tapa dura - 2003
de Alan V. Smrcka (Editor)
Primera línea
The purification of recombinant G protein a subunits expressed in Escherichia coli (E. coli) is a convenient and inexpensive method to obtain homogeneous preparations of protein for biochemical and biophysical analyses.
Descripción de contraportada
G proteins and G protein-coupled receptors are ubiquitously expressed proteins that regulate a wide range of physiological processes and are consequently the targets of many pharmaceuticals. In G Protein Signaling: Methods and Protocols, leading researchers describe in detail key methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. The authors focus on the mechanisms of G protein and G protein-coupled receptor function and the roles of G protein subunits in cell biology and disease. Among the readily reproducible techniques presented are those for the purification of G proteins and effector enzymes, assays of these purified G proteins and effector enzymes, and for the study of G protein interactions with effectors in intact cells. Additional methods are provided for assaying G protein-coupled receptor structure, function, and localization, for studying the physiological roles for endogenous G proteins, and for examining lipid and phosphate modifications of RGS proteins. Each fully tested protocol includes a background introduction explaining the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
Comprehensive and highly practical, G Protein Signaling: Methods and Protocols offers novice and expert researchers alike an array of cutting-edge tools to illuminate the roles of G protein and G protein-coupled receptors, as well as a better understanding of their action with drugs, neurotransmitters, and sensory stimuli.
Detalles
- Título G Protein Signaling: Methods and Protocols
- Autor Alan V. Smrcka (Editor)
- Encuadernación Tapa dura
- Edición U. S. EDITION
- Páginas 250
- Volúmenes 1
- Idioma ENG
- Editorial Humana
- Fecha de publicación 2003-09-15
- Ilustrado Sí
- Features Bibliography, Illustrated, Index
- ISBN 9781588291370 / 1588291375
- Peso 1.38 libras (0.63 kg)
- Dimensiones 9.18 x 6.22 x 0.95 pulgadas (23.32 x 15.80 x 2.41 cm)
- Library of Congress subjects Cellular signal transduction, G proteins
- Número de catálogo de la Librería del Congreso de EEUU 2003042323
- Dewey Decimal Code 572.643
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G PROTEIN SIGNALING: METHODS AND PROTOCOLS (METHODS IN MOLECULAR BIOLOGY) VOL 237
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G PROTEIN SIGNALING: METHODS AND PROTOCOLS (METHODS IN MOLECULAR BIOLOGY) VOL 237
de ALAN V. SMRCKA,
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G Protein Signaling: Methods and Protocols (Methods in Molecular Biology, 237)
de Smrcka, Alan V. [Editor]
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San Diego, California, United States
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